组织蛋白酶B和基质金属蛋白酶-9在喉癌中的表达

Expression of Cathepsin B and MMP-9 in Laryngeal Squamous Cell Carcinoma and Its Significance

目的:通过检测人喉癌组织中组织蛋白酶B(Cathepsin B,CB)和基质金属蛋白酶-9(MMP-9)的表达,探讨其在喉癌中的表达及其与淋巴结转移的关系。方法:采用免疫组织化学(S-P)法,检测41例人喉癌标本、20例正常喉黏膜标本(对照组)中CB和MMP-9的表达。结果:CB和MMP-9在喉癌组织中的表达高于对照组(P<0.01),在有淋巴结转移的喉癌组织中的表达高于无淋巴结转移喉癌组织(P<0.05);CB和MMP-9的表达之间存在明显的相关性。结论:喉癌中CB、MMP-9的表达可能是癌肿发生、生长和浸润转移的重要因素,可作为判断肿瘤恶性程度和预后的重要指标。

Objective： To investigate the expression of cathepsin B, matrix metalloproteinase-9 （ MMP-9 ） in laryngeal carcinoma, and its correlation with lymph node metastasis. Methods： Using immunohistochemistry method, the expressions of these two proteases in 41 cases of laryngeal cancer and 20 cases of normal laryngeal cavity mucosa （ as control） were examined. Results： The expression of cathepsin B and MMP-9 in laryngeal carcinoma tissues were higher than those in control （ P 〈 0.05 ） ; and their expressions were even higher in laryngeal carcinoma cases with lymph node metastasis than those in cases without metastasis （ P 〈 0.05 ）. There was also a significant correlation in staining intensity between cathepsin B and MMP-9. Conclusions：The expressions of cathepsin B and MMP-9 increase in laryngeal carcinoma which might be important factors involved in carcinogenesis, tumor development, invasion and metastasis. Their expression can be used as predictors of malignant behavior and prognosis of laryngeal carcinoma.