利福霉素产生菌育种及发酵罐放大试验研究

Study on Breeding and Scaleup in Fermenters of Rifamycin Producer

用紫外和氯化锂复合诱变利福霉素产生菌,得到发酵效价为6837 u/mL的菌株A22-04,比初始菌株提高36.5%。对该菌株进行发酵罐放大试验,10 L罐发酵效价为7234 u/mL,发酵时间为120 h,比对照菌株缩短了12 h;30 m^3生产罐的发酵效价为6497u/mL,发酵时间也略有缩短。

Using UV and LiCl as mutating agent, the Amycolatopsis mediterranei strain A22-04 was successfully obtained. The strain＇s potency of rifamycin SV recheached 6 837 u/mL and was 36.5% higher than the original strain. In 10 L fermentor its potency was 7 234 u/mL and the fermentation period was 120 h ,with 12 h shorter than the former fermentation time. And in 30 m^3 tank its yield was 6 497 u/mL and the fermentation time was little shorter than the original.