超滤用于茶树花多酚纯化工艺的研究

Studies on the Purification of Polyphenols from Tea Plant Flower by Ultra-filtration

以茶树花为原料,超滤处理茶树花多酚乙醇漫提液,得出适宜超滤条件:选择截留分子质量20～50ku超滤膜组件,操作温度30℃、超滤压力0.10 MPa、多酚浓度4.0 mg/mL,后期采用加水透析超滤;在此条件下,蛋白质截留事为73.94%,经后续吸附树脂纯化多酚的吸附容量增加了35.8%,样品纯度达到84.3%,比未超滤纯度提高了32.8%;茶树花多酚HPLC色谱图分析,超滤去杂以后,多酚中的儿茶素类组分得到了充分富集,说明超滤用于树脂法纯化茶树花多酚工艺的有效性。

The ethanol extract of tea plant flower polyphenols was purified by ultra-filtration （UF） in different conditions. The optimal UF parameters were found to be a membrane assembly with a weight molecular cut-off 20-50 ku, an operation temperature at 30℃, under a filtration pressure of 0.10 MPa. The initial polyphenol concentration was 4.0 mg/mL, while the later stage of UF separation was enhanced by addition of water for dialysis. The UF separation retained 73. 94% of proteins in the extract. This increased the absorption capacity of the absorbent resin by 35.8% during the following stage. Therefore, the purity of the polyphenols after resin adsorption reached 84.3% for the UF treated extract, compared to 32.8% for the extract without UF. The HPLC chromatograms also showed that catechins were sufficiently enriched after UF, indicating the efficiency of UF treatment for purification of polyphenols by absorbent resins.