小檗碱对大鼠肠黏膜微血管内皮细胞分泌一氧化氮的影响

Effect of Berberine on Nitric Oxide Release of Rat Intestinal Mucous Microvascular Endothelial Cells

目的研究小檗碱对体外培养大鼠肠黏膜微血管内皮细胞分泌一氧化氮(NO)的影响,探讨小檗碱治疗肠道疾病的药理作用机制。方法体外培养大鼠肠黏膜微血管内皮细胞,采用第Ⅷ因子相关抗原免疫荧光染色进行鉴定,在培养3、6、9、12h后,用比色法检测正常细胞与不同浓度小檗碱处理细胞上清液中NO含量,比较各组细胞NO的分泌水平。结果小檗碱处理组NO水平显著高于正常对照组,以5μg/mL剂量组作用最显著。结论促进内源性NO释放,介导肠黏膜微血管内皮依赖性舒张反应,改善肠道局部微循环,是小檗碱一个重要的药理作用机制。

Objective To study the effect of berberine on the release of nitric oxide （NO） by rat intestinal mucous microvascular endothelial cells （RIMECs） cultured in vitro for exploring the pharmacological mechanism of berberine in treating intestinal disease. Methods Cultured RIMECs in vitro were identified adopting immunofluorescent stain with factor Ⅷ-related antigen. NO level in the supernatant of normal cell culture or cell culture treated with different concentrations of berberine was detected with colorimetry at the 3rd, 6th, 9th and 12th h and compared. Results Compared with that in the normal control group, NO level increased more obviously in the berberine treated groups, and the best effect was shown in the 5 μg/mL berberine treated group. Conclusion One of the important pharmacological mechanisms of berberine might be through promoting the endogenous NO release to induce endothelium-dependent dilatation of microvascular in intestinal mucosa, thus to improve the local microcirculation of intestine.