NAPP体外抗乙型肝炎病毒作用的实验研究

EXPERIMENTAL STUDY ON THE EFFECT OF PROTOPORPHYRIN DISODIUM IN VITRO ON ANTI-HBV

[目的]应用HepG2.2.15细胞株作为体外抗乙型肝炎病毒的实验模型,研究NAPP对乙肝病毒的抑制作用。[方法]以NAPP梯度浓度与HepG2.2.15细胞混合培养,通过MTT法检测药物的细胞毒性,9 d后取细胞培养上清液,用ELISA法测定HBsAg、HBeAg含量,荧光定量PCR检测上清液中病毒载量。[结果]NAPP浓度在1 mg/ml时,对细胞无明显毒性;NAPP在所稀释的各浓度下,对HBsAg、HBeAg均有抑制作用,对HBsAg、HBeAg的治疗指数分别大于188.7和64.5;NAPP可抑制HBV-DNA的复制。[结论]NAPP体外对HBV有显著的抑制作用。

[Objective]To study the in-vitro inhibitation of Hepatitis B virus of NAPP and the cytotoxicity to HepG2.2.15 cell strain.[Methods]The cell toxicity of NAPP was determined with MTT method,and the effect of NAPP on HBsAg and HBeAg extracted by HepG2.2.15 cells was determined under diffenent concentrations on the ninth day with ELISA method.Real-time fluorescence quantitative PCR was used for determining extracted HBV-DNA in the culture medium.[Results]NAPP had no obvious cell toxicity at the concentration of 1mg/ml and depressed duplication of HBV DNA.NAPP had obvious restrain effect on HBsAg and HBeAg.The therapeutic index of NAPP was 188.7 for HBsAg and 64.5 for HBeAg.[Conclusion]NAPP has no obvious immediate cell toxicity on the cells and can inhibit the duplication of Hepatitis B Virus in Vitro.