雷公藤甲素对KA损伤大鼠星形胶质细胞的影响

Effects of Triptolide on Astrocytes Induced by KA Injection Into Rat Hippocampus

目的:观察海人藻酸(Kainic acid,KA)海马内注射后星形胶质细胞的变化及雷公藤甲素(TRP)对其的影响。方法:90只SD大鼠(200～220g)随机分为3组:右侧海马注射生理盐水后生理盐水灌胃作为对照组(NS+NS),右侧海马注射海人藻酸后生理盐水灌胃干预组(KA+NS),右侧海马注射海人藻酸后雷公藤甲素灌胃干预组(KA+TRP)。动物存活1天,3天,5天,7天,14天后免疫组织化学结合图像分析技术观察海马内星形胶质细胞形态和数目的变化。结果:(KA+NS)组海马内星形胶质细胞数目明显增多,胞体明显增大,突起变短,变粗,与(NS+NS)组相比差别具有显著性(p<0.05);(KA+TRP)组星形胶质细胞数量明显减少,胞体变小,突起变细长,与(KA+NS)组相比差别具有显著性(P<0.05)。结论:KA注射后可导致大鼠海马内星形胶质细胞的激活,雷公藤甲素对KA诱导的星形胶质细胞的活化有抑制作用。

Objective： To observe changes of astrocytes in hippocampus of rats under the injection of Kainic acid and the effect of triptolide （TRP） on them. Methods： 90 SD rats were divided randomly into three groups： NS treated after NS injection into the right dorsal hippocampus group （NS＋NS, n=30）; NS treated after KA injection into the right dorsal hippocampus group （KA＋NS, n=30）; TRP treated after KA injection into the right dorsal hippocampus group （TRP＋KA, n=30）. The animals were sacrificed at 1d, 3d, 5d, 7d, 14d after KA injection. The histochemical method and image-analysis technique were used to detect the changes of the number and morphology of astrocytes. Results： In KA＋NS group, the number of the astrocytes in hippocampus increased significantly, and the cell bodies got thicker and the processes became shorter and thicker, compared with NS＋NS group （p〈0.05）. In TRP＋KA group, the number of astrocytes decreased and the cell bodies became smaller and the processes got longer and thinner than those of KA＋NS group （P〈0.01）. Conclusion： The injection of KA into the hippocampus results in the activation of astrocytes and the treatment of TRP can rescue the effect of KA on astrocytes.