利巴韦林鼻用温敏凝胶的鼻腔纤毛毒性研究

Study on the ciliotoxicity of thermoreversible nasal gel of ribavirin

目的:研究利巴韦林鼻用温敏凝胶及其处方中原料和辅料对鼻腔纤毛运动的影响。方法:采用在体蟾蜍上颚模型法,分别给予原料药利巴韦林(0·5%),辅料泊洛沙姆407(20%)、泊洛沙姆188(2·0%)、聚乙二醇6000(2·0%)、苯扎氯氨(0·01%)和自制的利巴韦林鼻用温敏凝胶,检测纤毛持续摆动时间,显微镜下观察纤毛形态学改变。结果:利巴韦林原料(0·5%)、泊洛沙姆407(20%)、泊洛沙姆188(2·0%)、聚乙二醇6000(2·0%)、苯扎氯氨(0·01%)及利巴韦林鼻用温敏凝胶均对鼻腔纤毛运动无明显影响,相对于生理氯化钠溶液,纤毛持续摆动时间的相对百分率在87·9%～97·6%之间。结论:利巴韦林鼻用温敏凝胶无明显鼻腔纤毛毒性,可以用于鼻腔给药。

Objective：To study the ciliotoxicity of thermoreversible nasal gel of ribavirin and the component in this formulation. Methods：The lasting time of ciliary movement was determined and the ciliary morphology was observed with microscope after administration in situ toad palate model. Results： All of 0. 5% ribavirin,20% P407,2.0% P188,2.0% PEG6000,0. 01% BKC and thermoreversible nasal gel of ribavirin had no significant effect on ciliary movement. The relative percentage of the lasting time of ciliary movement with physiological was 87.9% -97.6%. Conclusion：In this study the thermoreversible nasal gel of ribavirin has no significant ciliotoxicity and it can be used for intranasal administration.