茵陈蒿汤对乙醇诱导的大鼠急性肝损伤的保护作用研究

Hepatoprotection of Yin Chen Hao Tang on Acute Rat Liver Injury Induced by Ethanol

目的:系统评价茵陈蒿汤对乙醇诱导的大鼠急性肝损伤的保护作用。方法:75只Wistar大鼠随机分为5组,模型组给予生理盐水,给药组按体重分别给予茵陈蒿汤冻干粉,连续给药14天,于第10日起模型组和给药组同时灌胃给予乙醇50%(v/v)连续5天(5g/kg),空白组给予生理盐水;于第15日处死大鼠,分离血清及肝组织匀浆液,检测谷丙转氨酶(ALT)、谷草转氨酶(AST)、甘油三酯(TG)、乙醇脱氢酶(ADH)、γ-L-谷氨酰转肽酶(γ-GT)、谷胱甘肽(GSH)和丙二醛(MDA)的活性和效价,观察肝脏组织学情况。结果:模型组血清ALT、AST、ADH、γ-GT及TG活性明显高于正常对照组,有显著性差异(P<0.01),表明模型复制成功;与正常对照组相比,治疗组上述指标仅轻度升高,与模型组比较有显著性差异(P<0.01);模型组MDA含量明显高于正常对照组,具有显著性差异,治疗组低剂量和中剂量也明显升高,与模型组比较差异无显著性意义(P<0.05);治疗组高剂量轻微升高,与模型组比较有显著性差异(P<0.01);模型组GSH含量明显低于正常对照组,有显著性差异(P<0.01),其在治疗组低剂量和中剂量中也明显降低,与模型组比较无显著性差异,而在治疗组高剂量中轻微降低,与模型组比较有显著性差异(P<0.01),同时,组织病理学也得出上述相同的结论。结论:茵陈蒿汤对乙醇诱导的大鼠急性肝损伤具有良好的保护作用。

This paper evaluates hepatoprotective effects of Yin Chen Hao Tang on acute rat liver injury induced by ethanol. In the investigation, seventy - five rats were randomly divided into five groups, including control group （CG） , ethanol treated group （ EG）, low dose group of Yin Chen Hao Tang （ LG）, middle dose group of Yin Chen Hao Tang（MG） and high dose group of Yin Chen Hao Tang（HG）. The LG, MG and HG were treated with frozen dry powders of Yin Chen Hao Tang at a dosage of 2g/kg/d,6g/kg/d and 12g/kg/d in line with body weight for fourteen days in a row. The LG, MG and HG were orally administrated with 50% ethanol（v/v）at a dosage of 5g/kg, 2.5 hours after YCHT administration, starting from day ten for five consecutive days. The EG were orally administrated with 50% ethanol（v/v）at a dosage of 5g/kg for fourteen consecutive days. The CG received distilled water in an equal amount. On the fourteenth day, all blood samples and liver tissues were collected from the rats under anesthesia for experimental analysis and histological slicing, 12 hours after final ethanol administration. It is found that the levels of ALT and AST were significantly （ （P 〈 0. 01） ） elevated after ethanol administration, when compared with the CG. The treatment of Yin Chen Hao Tang at a dosage of 2g/kg, 6 g/kg and 12 kg showed a significantly decreased level （（P 〈0.01 ）） of ALT and AST, compared with the EG. However, the serum level of ADH, γ - GT and TG was also significantly （ （ P 〈 0.01 ） ） elevated after ethanol administration, compared with the CG. The treatment of Yin Chen Hao Tang at a dosage of 2g/kg, 6 g/kg and 12 kg showed a significantly decreased level （P≤0.05） of γ- GT, TG and ADH against the EG. Rats administered with ethanol alone were found significantly （ （P 〈 0.01 ） ） lowered in their hepatic GSH levels. The treatment of Yin Chen Hao Tang at a dosage of 12 g/kg exhibited a significantly increased （ （P 〈0.01 ） ） hepatic GSH level, though the Yin Chen Hao Tang at a dosage of 2g/kg and 6 g/kg simply did not prevent the dropping of hepatic GSH levels, compared with the EG. A significant increase （ （P 〈0.01 ） ） of liver tissue MDA level was observed in the EG. However, ethanol induced elevation of MDA levels came down significantly （ （ P 〈 0. 01 ） ） after the treatment of rats with Yin Chen Hao Tang at a dosage of 12g/kg. Application of Yin Chen Hao Tang at a dosage of 2g/kg and 6 g/kg did not show a lowered MDA against the EG. The same result was also seen in histological observations. The preliminary investigation has shown an effective hepatoprotectivity of Yin Chen Hao Tang on acute rat liver injury induced by ethanol.