不同产地栀子药材HPLC指纹图谱研究

Fingerprints of Gardenia Jasminoides Ellis in Different Habitats

采用高效液相色谱(HPLC)方法建立了江西道地药材栀子的HPLC指纹图谱,并将不同产地的栀子药材进行了指纹图谱相似度计算,为科学评价和有效控制栀子质量提供了新方法。色谱条件:C_(18)柱(250mm×4.6mm,5μm),流动相为乙腈-水,检测波长为258nm,体积流量为0.8mL/min,柱温30℃;线性梯度洗脱。该方法能简便、快速地鉴别和区分不同来源的栀子药材,为全面控制栀子药材的质量提供了依据。

Authors established HPLC fingerprints for Gardenia jasminoides Ellis collected from Jiangxi province, and compared them with Gardenia jasminoides Ellis grown in other habitats. HPLC fingerprints of Gardenia jasminoides Ellis were collected from eight different places. HPLC separation was performed on a Diamonsil^TM C18 analytical column gardenia, eluted with acetonitrile -water at a flow rate of 0.8mL/min. The temperature of column was set at 30%, with UV detection wavelength at 258nm. The HPLC fingerprints were established, and compared with similar Gardenia jasminoides Ellis grown in other habitats. The method has been proved simple and rapid in identifying and differentiating Gardenia jasmionides Ellis collected from different places, with a secured quality control.