RP-HPLC法测定乙烷硒啉分散片中药物的含量

A RP-HPLC method for determination of ethaselen dispersible tablets

目的:建立乙烷硒啉分散片含量测定方法。方法:采用反相高效液相色谱法,使用BDS HYPERSILC18柱(250mm×4.6mm,5μm),检测波长为320nm,柱温为室温,流动相为0.01%磷酸-甲醇(60∶40),流速1.0mL.min-1。结果:溶剂与辅料对分散片中药物的含量测定无干扰,乙烷硒啉在10～400μg.mL-1范围内呈良好的线性关系(r=0.9995),精密度RSD为0.39%;日内精密度为0.57%;平均回收率为101.2%(n=9)。结论:该方法专属性强,操作方便,结果准确,重现性好,可用于乙烷硒啉分散片中药物的含量测定。

Objective：To establish a RP-HPLC method for assaying ethaselen dispersible tablets. Methods：The chromatographic separation was performed on a BDS HYPERSIL C18 column （250 mm×4.6 mm, 5μm） at room temperature. The mobile phase consisted of CH3OH and 0. 01% H3PO4 （40： 60）. Flow rate was 1.0 mL·min^-1, with detection at 320 nm. Results：Calibration curve for eth aselen ranged from 10 to 400μg-mL^-1 （r =0. 999 5 ）. The average recovery was 101.2% （ n =9）. Conclusion ：A simple, accurate, and specific RP-HPLC method for assaying ethaselen dispersible tablets is established.