HPLC法测定骨刺平片中柚皮苷的含量

Determination of the Content of Naringin in Guciping Tablet by HPLC

目的:采用高效液相色谱法测定骨刺平片中柚皮苷的含量。方法:Alltech Apollo C18色谱柱(250mm×4.6mm 5μm):流动相:乙腈-2%冰醋酸(22:78);流速:1.0mL/min:检测波长为283nm。结果:柚皮苷线性范围为0.08～0.48μg.平均回收率为99.47%,RSD=1.7%(n=6).结论:所建方法简便、准确、重复性好,可用于骨刺平片的质量控制。

Objective：To establish a method for determining Naringin in Guciping tablet. Methods： HPLC, the Alltech Apollo C18 column （4.6mm × 250mm, 5μm） was used, acetonitrile - 2%acetic acid （22 ： 78） as mobile phase, the detection wavelength was at 283nm. Results： The standard curve was linear within a range of 0.1～40μg.The average recovery and RSD were 99.47 % and 1.7 % respectively （n = 6）. Conclusion ： This method is accurate, reliable and provides a scientic index for controlling the quality of Guciping.