侧柏树干的超临界CO_2萃取物组分分析及双条杉天牛的EAG反应

Chemical analysis and electroantennogram responses in Semanotus bifasciatus adults to Platycladus orientalis volatiles extracted by SFE

利用超临界CO2萃取法和索氏提取法分别提取侧柏Platycladus orientalis(L.)的韧皮部和木质部挥发物,经GC/MS分析其成分,结果表明:超临界CO2萃取物中检测到的21种组分中以倍半萜烯及其氧化物为主,没有检测到单萜类物质;索氏提取法检测到23种组分,韧皮部中检测到的组分以高沸点的树脂酸和二萜等为主,单萜烯和倍半萜及其氧化物含量较低;木质部中以倍半萜烯及其氧化物为主。将不同提取样品进行双条杉天牛Semanotus bifasciatus(Motschulsky)的触角电位反应,结果表明:2种方法的萃取物均能引起天牛的EAG反应,但以超临界萃取物引起的EAG反应较强,且与索氏提取物的反应值差异显著。罗汉柏烯和雪松醇可能是侧柏植物中引诱双条杉天牛的重要活性组分。

The volatiles from phloem and xylem of Platycladus orientalis （ L. ） were extracted by supercritical fluid extraction （SFE） and sohlet＇s extraction methods （SEM）, and analyzed by GC-MS. For extracts by SFE, 21 chemicals were identified by GC/MS, among them sesquiterpenes and their oxides composed as the major part, whereas monoterpenes were not detected. For SEM, 23 chemicals were identified. In the phloem volatiles, the high- boiling point chemicals such as resin acids and diterpenes were the major part with very little monoterpenes and sesquiterpenes. However, in the xylem volatiles, sesquiterpenes and their oxides composed the major part also. Semanotus bifasciatus （Motschulsky） had electroantenogram （EAG） responses to both extracts by SFE and SEM, whereas the responses to SFE＇s extracts were much stronger than to SEM＇s, and their differences were statistically significant. So, the thujopsene and cedrol from P. orientalis may be the active component to attract S. bifasciatus.