高效液相色谱法测定化妆品中邻苯二甲酸二丁酯和邻苯二甲酸二(2-乙基己基)酯的含量

Determination of dibutyl phthalate and bis (2-ethylhexyl) phthalate in cosmetics by high performance liquid chromatography

目的：建立了高效液相色谱法测定化妆品中邻苯二甲酸二丁酯（DBP）和邻苯二甲酸二（2-乙基己基）酯（DE-HP）含量的方法。方法：采用固相萃取法处理样品，淋洗试剂为甲醇：乙醚=1：19。甲醇和水作为流动相，二极管阵列检测器测定DBP和DEHP。结果：DBP测定的线性范围为5．0～200μg／ml，线性相关系数为0．9993，DEHP测定的线性范围为5．5～220μg／ml，线性相关系数为0．9997。DBP的回收率范围在84％～93％，相对标准偏差为3．2％～4．8％。DE-HP的回收率范围在98％～105％，相对标准偏差为2．8％～4．7％。结论：该方法灵敏度高，结果准确而且稳定。

Objective：A method for the determination of DBP and DEHP in cosmetics by HPLC was established. Methods： Samples were extracted by solid phase extraction, and eluting solvent was methanol： ethyl ester = 1：19. Methanol and water we applied as mobile phase, DBP and DEHP were determined by PDA detector. Results： The linear ranges of method, linear correl tion coefficients, recovery rates and relative standard deviations were 5. 0 -200μg/ml, 0. 9993, 84%-93% and 3. 2% -4. 8% for DBP and 5.5 -220μg/ml,0. 9997, 98% -105% ,2. 8% -4. 7% for DEHP,respectively. Conclusion：The method is high se： sitive, accurate and highly reproducible, and is suitable for the determination of DBP and DEHP in cosmetics.