高效液相色谱法同时测定食品成品中的对位红和苏丹红

Study of a analytical method for detecting simultaneously para red and Sudan dyes in foodstuffs

目的：建立食品成品中简便、易行的同时检测对位红和苏丹红系列的高效液相色谱方法。方法：样品中的对位红和苏丹红经正己烷提取、采用光谱分析选择合适测定波长、梯度洗脱、C18柱分离，外标法定量。结果：对位红、苏丹红I、Ⅱ最低检测限为0．3mg／ks，在0．3～20ms／kg范围内呈良好的线性关系。苏丹红Ⅲ、Ⅳ最低检测限为0．05ms／kg，在0．05—20mg／kg范围内呈良好的线性关系。5种色素相关系数均≥0．995，平均回收率在76．9％～127．0％范围内，RSD≤10％（n=6）。结论：采用本方法同时完成食品成品中对位红、苏丹红I、Ⅱ、Ⅲ、Ⅳ的测定，不需要固相萃取，从而简化样品前处理步骤，优化了色谱条件，缩短了测定时间。

Objective：A simple and fast analytical method for detecting simultaneously pant red, Sudans I, II, III, and IV in foodstuffs by high - performance liquid chromatography is described. Methods： These dyes were extracted from the samples with n - Hexane and analyzed by gradient elution. The chromatographic separation was carried out on a reverse phase C18 column. This paper presented a simple analytical method to identify and quantify simultaneously para red Sudans I, II, III, and IV by external reference method, and to select detected wavelength by spectroscopic analysis. Results：Calibration curves were linear in the 0. 3 - 20 mg/kg range for para red and Sudans I, II and in the 0. 05 - 20 mg/kg range for spices Sudans III, and IV. Limits of detection were 0. 3 mg/kg for pant red and Sudans I, II and 0. 05 mg/kg for Sudans III, and IV. The overall recoveries were in the range of 76.9% - 127. 0% depending on the dye involved. Validation data showed a good repeatabilityand within - lab reproducibility with relative standard deviations ≤ 10%. Condusion：The proposed method is specific, selective and fast. Samples don＇t need SPE （solid phase extract） ,thereby reduce extraction procedure, The method shorten detection time by optimizing chromatographic separation condition.