摘要
目的:对甲鱼进行霍乱弧菌检测,为疾病预防控制提供准确可靠的试验数据。方法:按照卫生部《霍乱防治手册》第五版检测方法。同时,用霍乱弧菌快速层析试剂检测和科玛嘉弧菌显色培养基分离培养。结果:从24份甲鱼体表涂抹物中用霍乱弧菌快速层析试剂检测出2株O1群霍乱弧菌,用科玛嘉弧菌显色培养基和庆大琼脂分离出3株O1群霍乱弧菌。噬菌体-生物分型为31L型,常见非流行株。结论:本次监测结果(3/24)阳性率较高(12.5%),建议加强海产品、水产品霍乱弧菌监测。
Objective：To provide accurate reliable test data for disease control and prevention by detecting Vibrio cholera from turtle surface. Methods： Vibrio cholera was detected according to ministry of health （ Vibrio cholera combat manual） fifth edition and Vibrio cholera gold - immunochromatography assay （GICA） and CHROMAGAR vibrio chromogenic medium. Results： Results of detecting Vibrio cholera in 24 turtle surface showed respectively that two samples of group O1 Vibrio cholera was positive by using GICA＇s detection methods and three samples of group O1 Vibrio cholera was positive by using CHROMAGAR vibrio chromogenie medium and Big - agar medium. Phage - biological classification was 31 typing, common the non - epidemic strains. Conduslon：This monitoring results （3/24） shows that positive rate is higher （12. 5% ） and recommend strengthening monitoring Vibrio cholera in seafood and aquatic products.
出处
《中国卫生检验杂志》
CAS
2007年第9期1685-1686,共2页
Chinese Journal of Health Laboratory Technology
关键词
霍乱弧菌
甲鱼
检验方法
Vibrio cholera
Turtle
Detection method
