HPLC法测定甲麻芩苷那敏片中三组分含量

Determinate of Three Composition of Methylephedrine Hydrochloride,Baicalin and Chlorphenamine Maleate Tablets by HPLC

建立反相离子对HPLC法测定甲麻芩苷那敏片中三组分的含量。采用Diamonsil C18色谱柱,以[0.2%辛烷磺酸钠溶液-三乙胺（100∶0.2,用磷酸调节pH值为4.3）]-甲醇（50∶50）和（40∶60）为流动相,分别测定盐酸甲麻黄碱、黄芩苷及马来酸氯苯那敏,检测波长为215nm,流速为1.0 mL·min^-1。盐酸甲麻黄碱、黄芩苷及马来酸氯苯那敏的线性范围分别为16～125μg.mL-1（r=0.9999）、11～85μg·min^-1（r=1.000）及26～102μg·min^-1（r=0.9991）,平均回收率分别为99.7%（RSD=1.1%）、99.4%（RSD=1.1%）及100.2%（RSD=1.6%）。本方法简单、迅速、准确,可用于甲麻芩苷那敏片中三组分的含量测定。

A RP- Ion pair HPLC method for Hydrochloride, Baicalin and Chlorphenamine Maleate assay of three composition of Methylephedrine Tablets was established. The method was carried out on the diamonsil C18 Column, [0.2% sodium Octanesulfonate- triethylamine （100 ： 0.2, adjust pH value to 4. 3 with phosphoric acid）] -methanol （50 ：50） and （40 ： 60） was used as the mobile phase, to determine Methylephedrine Hydrochloride. Baicalin and chlorphenamine Maleate, respectively. The detection wavelength was 215 nm and flow rate was 1.0 mL ·min^-1. The linear range for Methylephedrine Hydrochloride. Baicalin and chlorphenamine Maleate were 16～125μg·min^-1（r=0. 9999）. 11-85μg·min^-1 （r= 1. 000） and 26～102μg·min^-1（r= 0. 9991）, respectively. The average recoveries rates of method were 99.7% （RSD=1.1%）. 99.4% （RSD=1.1%） and 100.2% （RSD=1.6%） respectively. The method is simple, quick and accurate, it can be used for quality control of three composition in the drug.