慢性非细菌性前列腺炎患者前列腺液16SrRNA基因的检测及其临床意义

Detection of 16 SrRNA gene in EPS of patients with CPPS and clinical implication

目的探讨应用聚合酶链反应(PCR)检测慢性非细菌性前列腺炎(CPPS)患者前列腺液(EPS)中细菌基因,并与CPPS患者的临床治疗效果做相关性分析。方法以16SrRNA基因为靶序列,设计引物及寡核苷酸探针,采用PCR法检测标准菌株及135例CPPS患者前列腺液中细菌基因。所有CPPS患者均应用抗生素和中成药规范治疗,每2周为1疗程,每2周复查EPS常规1次,必要时适当调整药物。治疗3个月时,进行疗效评估。结果135例CPPS患者中16SrRNA检测结果阳性为78例,阳性率为57.78%。16SrRNA阳性组患者有效率84.6%,阴性组有效率52.6%;16SrRNA阳性组患者疗效明显优于阴性组。结论大部分CPPS患者的前列腺液中可以检测到细菌的16SrRNA基因,提示细菌感染在CPPS的发病中有重要作用。

Ojective To study the correlation of 16S rRNA gene detected by PCR in EPS of patients with CPPS and clinical curative effect. Methods The 16S rRNA in EPS of 135 patients with CPPS was detected by PCR. The patients were randomized to receive quinolones like Lelang, Woerde medicines and Chinese formulated products like Ningmitai, Wonglitong. After treatment for 3 months, the curative effect was evaluated with NIH-CPSI and compared with that before therapy. The SPSS Chi-Square test and logistic regression analysis program were used for statistic analysis. Results Of the 135 CPPS patients, positive 16SrRNA signals were detected in 78 samples and negative in 57 samples respectively. The positive 16SrRNA signals were detected in 3 samples of the health controls and negative in 18 samples respectively, There was significant difference in the positive rate between CPPS group and health control group. Curative effect in the patients positive for 16SrRNA signal was better than in those negative for 16SrRNA. Conclusion Bacteria 16 SrRNA can be detected in the EPS of most CPPS patients, suggesting that bacteria infection may play an important role in chronic prostatitis.