调脂护肝方对高脂饲养所致脂肪肝大鼠生化指标的影响

Therapeutic effect of Tiaozhi Hugan decoction treatment on the biochemical indicator of the rat's fatty liver caused by fatty diet

目的观察调脂护肝方对高脂饲食脂肪肝大鼠的干预效应,探讨其药效学机制。方法将雄性SD大鼠64只正常喂养1周后,随机分为正常组12只饲基础饲料,实验组52只给予高脂饲料饲食(含胆固醇2%,猪油10%,基础饲料88%),13周末随机抽取正常组2只、高脂饲养8只,验证高脂饲养大鼠脂肪肝形成。剩余44只随机分为4组即病理模型组、东宝甘泰组、羊栖菜组、调脂护肝方组,每组11只。在继续饲高脂饲料外,分别给予东宝甘泰,按0.5g/(kg.d)(用蒸馏水配成0.05g/ml的混悬液),羊栖菜煎剂按10ml/(kg.d)(10ml含羊栖菜生药20g),调脂护肝方按10ml/(kg.d)(由羊栖菜、丹参、制大黄、葛根、泽泻、茯苓=3:2:1:1:1:1),共8周。第21周末称重,处死动物,取肝脏,计算肝脂数(肝重/体重×100%),抽血,并检测:血清总胆固醇(TC)、甘油三酯(TG)、高密度脂白蛋白-胆固醇(HLD-C)、血清丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)、血清超氧化物歧化酶(SOD)、丙二醛(MDA)。结果与病理模型组、东宝甘泰组比较调脂护肝方组大鼠体重、肝指数血清TC值、TG值均降低,HLD-C值增高,血清ALT、AST、MDA值均较低,SOD值较高(各组比较P<0.01)。结论调脂护肝方干预非酒精性脂肪肝的药效机制为:降低体重与肝指数,调整脂质代谢,改善肝功能,抗脂质过氧化。

Objective To observe theraputic effect of Tiaozhi Hugan decoction treatment and investigate its pharmcodynamic. Methods 64 SD male rats were fed a normal diet for one week then they were randomly divided into two groups ,one was normal group（which were 12 rats）fed normal diet, the other , which were 52 rats fed high fatty diet for 12 weeks. At the end of 13th week, 2 rats from fed normal diet group and 8 rats from fed high fat diet group were scarified to test fatty liver model .Then 44 rats,which were fed high fatty diet,were randomly divided into 4 groups of 11 each： model control group, DongBao GanTai group , seaweed decoction group and Tiaozhi Hugan decoction group. They were fed high fatty diet continuously for 8 weeks. At the same time model control group rats were given distilled water 10ml/（ kg·d） by gastrolavage , seaweed decoction group rats were fed seaweed decoction 20g/（ kg·d）. Dougbao Gantai group rats were fed Doug Bao Gantai, 0.5g/（kg·d）, Tiaozhi Hugan decoction group were fed Tiaozhi Hugan decoction 10ml/（dg·d） （contained seaweed 20g/kg）. At the end of 21th week, all rats were scarified, body weight, liver index（ liver/body weight ratio）serum triglyceride （TG）, total cholesterol （ TC ）, high density lipoprotein - cholesterol, （HLD - C ）, alartine aminotransferase （ALT）, aspartate aminotransferase, （,4ST）, superoxide dismutase（SOD） malonyl - dialdehyde （MDA） were assayed . Results Compared with model control group, DongBao Gantai group, the level of body weight, liver index, TC , TG, ALT, AST in serum in Tiaozhi Hugan decoction group were lower signifieantly . The level of HLD- C was higher., the level of MDA in Tiaozhi Hugan decection was lower significantly, the level of SOD was higher signifieantly（in all group P 〈 0.01 ）Conclusion It suggests that the pharmaeodynamies mechanisms of Tiaozhi Hugan Decoction to fatty liver induced by high fatty diet are lowering body weight and liver index, adjusting lipometabolism, improving liver function and having anti - lipid peroxidatien effects.