宫颈病变细胞核AgNOR颗粒形态定量分析

STUDYES ON NUCLEAR AND AGNOR MORPHOMETRY IN PRECANCEROUS AND CANCEROUS LESION OF THE CERVIX

为探讨不同程度宫颈病变的细胞核、ＡｇＮＯＲ的变化规律。利用自动图像分析技术分别对２８例宫颈癌，５５例宫颈非典型增生和１５例正常宫颈组织细胞核及ＡｇＮＯＲ颗粒形态定量参数进行测定。结果显示：除正常宫颈与轻度非典型增生宫颈组外，宫颈中度、重度非典型增生与宫颈鳞癌三组之间其平均细胞核面积、每个核ＡｇＮＯＲ颗粒数、平均颗粒面积、颗粒直径以及ＡｇＮＯＲ颗粒面积／细胞核面积之比值逐渐增加，而ＡｇＮＯＲ颗粒形状因子逐渐减小，各组之间存在着统计学差异。ＡｇＮＯＲ颗粒直径变化不显著。认为在宫颈组织发生癌变过程中，细胞核及核内ＡｇＮＯＲ颗粒大小、形态和数量均发生改变。

To investigate the value of nuclear and AgNOR morphometry as diagnostic indicators in cervical lesion. Methods: We measured nuclear and AgNOR morphometric parameters in 28 cases of cervix squamous epithelial cancer, 55 cases of cervical intracpithclial neoplasia and 15 cases of normal cervix by a computer assisted digitizing system. Result: From cervical intra epithelial neoplasia Ⅰ to cervix squamous epithelial cancer, mean nuclear area, mean AgNOR particle numbers per unclear, mean AgNOR particle area AgNOR particle diameter and particle area/nuclear area increased respectively, while AgNOR particle CRISF decreased. There were significant differences between each group. There were no significant differences in AgNOR particle perimeter. Conclusion: Once the cervical lesion progressed to cervix cancer, nuclear and AgNOR morphology changed dramaticlly. Nuclear and AgNOR morphometric parameters may be useful indicators for the malignant potentiality of cervious lesion.