RASSF1A和p16基因甲基化在子宫颈癌组织中的表达及意义

The expression and significance of hypermethylation of p16 and RASSF1A genes in cervical carcinoma tissues

目的:探讨p16和RASSF1A基因异常甲基化在子宫颈癌组织中的表达及意义。方法:用甲基化特异性聚合酶链反应法(methylation-specific PCR,MSP)对40例宫颈癌组织,80例宫颈上皮内瘤样病变(cervical intraepithelial neoplasia,CIN)组织、20例正常宫颈组织作为对照组进行p16和RASSF1A基因异常甲基化检测。结果:p16和RASSF1A甲基化在正常组未见表达。宫颈癌组p16基因甲基化阳性率为40.0%,明显高于CIN组的12.5%,差异有统计学意义(χ2=11.88,P<0.05)。宫颈癌组RASSF1A基因甲基化阳性率为20.0%,高于CIN组的7.5%,差异有统计学意义(χ2=4.04,P<0.05)。宫颈癌组p16和RASSF1A基因甲基化阳性率为55.0%,明显高于宫颈CIN组的17.5%,差异有统计学意义(χ2=17.12,P<0.05)。结论:p16和RASSF1A基因启动子区异常甲基化与宫颈癌的生物学行为相关,可能为宫颈癌的早期辅助诊断和治疗提供帮助。

Objective：To study the expression and significance of aberrant methylation of p16 and Ras association domain famlly A （RASSFIA） in cervical carcinoma. Methods：The cervical samples were divided into three groups： cervical carcinoma （CC） group （n = 40） , cervical intraepithelial neoplasia （CIN） group （n = 80） , and normal control group （n = 20 ）. The aberrant methylation of p16 and RASSFIA were detected by using methylation-specific reverse transcriptase polymerase chain reaction （MSP）. Results：Methylation of p16 and RASSFIA genes were not detected in normal control group. The positive rate of p16 gene methylation was significantly higher in CC group than that in CIN group （40.00% vs 12.5% ）. The difference was statistically significant （x^2 = 11.88, P 〈 0.05）. The positive rate of RASSFIA gene methylation was significantly higher in CC group than that in CIN group （20.0% vs 7.5% ）. There was significant difference （x^2 =4.04, P 〈0.05）. The positive rates of RASSFIA and p16 genes methylation were significantly higher in CC group than CIN group（55.0% vs 17.5% ）. The difference was significant （x^2 = 17. 12, P 〈 0.05）. Conclusion： Methylation status in the promoter regions of p16 and RASSFIA genes correlated with biological behaviors of cervical carcinoma. They may provide help in assistant diagnosis and treatment of cervical carcinoma.