rHuG-CSF动员健康供者外周血造血干细胞的效果观察

Observation on the mobilization of peripheral blood stem cells from healthy donors stimulated by rHuG-CSF

目的：观察重组人粒细胞集落刺激因子（recombinant human granulocyte-colony stimulating factor，rHuG．CSF）动员健康供者外周血造血干细胞的效果及影响因素。方法：将本研究中心异基因造血干细胞移植健康供者163例，分别采用3种不同厂家生产的rHuG-CSF进行外周血造血干细胞动员，对其动员效果、受者移植后造血重建等情况进行比较。结果：不同种rHuG-CSF动员后采集的单个核细胞（MNC）及CD34^＋细胞均能满足临床造血干细胞移植的需要，130例人类白细胞抗原（human leucocyte antigens，HLA）配型相合的同胞受者移植后均获得顺利植入。动员的单个核细胞数及CD34^＋细胞数与性别无关，而CD34^＋细胞数在41-60岁年龄组中明显减少（P〈0．05）；对采集时机的分析显示：第5天采集的单个核细胞数及CD34^＋细胞数最高（P〈0．05），此后逐渐下降。结论：糖基化的及两个非糖基化的rHuG-CSF制剂均能有效地在异基因移植中作为外周血造血干细胞动员剂。

Objective：To evaluate the effectiveness of recombinant human granulocyte-colony stimulating factor （rHuG-CSF） for stem cell mobilization from healthy donors and its related factors. Methods：For the matched 163 healthy donors rHuG-CSF was administered to stimulate mobilization of hematopoietic stem cells. The effectiveness of mobilization and hematopoietic reconstruction after allogeneie transplantation of peripheral blood stem cells （ PBSC ） were compared. Results： The yield of mononuclear cells （ MNC ） and CD34^＋ cells mobilized by different kinds of rHuG-CSF met the need of transplantation of hematopoietie stem cells in clinic. The PBSC were successfully transplanted into 130 human leukocyte antigens （HLA）-matched patients and satisfying hematopoietic reeonstitution was achieved. The numbers of MNC and CD34^＋ cell mobilized by rHuG-CSF were unrelated with sex. The number of CD34^＋cells decreased significantly in subjects aged between 41-60 years （P 〈 0.05 ）. The yield of MNC and CD34 ^＋ cells peaked at the fifth day after injection of rHuG-CSF （P 〈 0.05 ） and then decreased gradually. Conclusion： The one glyeosylated and two non glycosylated dosage forms of rHuG-CSF are all effective in stimulating mobilization of PBSC during allogeneic transplantation.