鲍氏不动杆菌脉冲场凝胶电泳和扩增片段长度多态性基因分型的研究

Molecular Typing of Acinetobacter baumannii by Pulsed Field Gel Electrophoresis and Amplified Fragment Length Polymorphism

目的利用脉冲场凝胶电泳(PFGE)和扩增片段长度多态性(AFLP)分型方法,了解医院鲍氏不动杆菌菌株亲缘性。方法采用NCCLS方法研究药物敏感性;采用PFGE和AFLP技术研究鲍氏不动杆菌基因型;对检测结果作聚合分析讨论菌株亲缘性。结果鲍氏不动杆菌利用PFGE分型可以分为4个亚型、利用AFLP分型可以分为2个亚型;聚合分析具有一致性。结论AFLP分型更具有实用价值。

OBJECTIVE To analyze the relationship of Acinetobacter baumannii in Ningbo No. 2 Hospital by pulsed field gel electrophoresis （PFGE） and amplified fragment length polymorphism （AFLP）. METHODS The sensitivity of 13 kinds antibacterials were analyzed according to American National Committee for Clinical Labotatory 2004＇s Standard. The genotypes were analyzed by PFGE and AFLP. The relationship was analyzed with cluster analysis methods. RESULTS Twenty seven strains of A. baumannii were divided into two groups by AFLP method or into four groups by PFGE method. Cluster analysis showed the concordance. CONCLUSIONS Amplified fragment length polymorphism has more clinical practice.