西沙必利联合腹膜氧合对油酸致急性肺损伤兔肺组织CC16 mRNA表达的影响

Effects of cisapride combined with transperitoneal oxygenation of Clara cell protein mRNA expression in the lung in a rabbit model of oleic-induced acute lung injury

目的评价西沙必利联合腹膜氧合对油酸致急性肺损伤兔肺组织CC16 mRNA表达的影响。方法家兔24只，雌雄不拘，体重2．0～2．5kg，用油酸诱导急性肺损伤模型成功后，随机分为4组（n=6）：急性肺损伤组（A组）、西沙必利组（B组）、腹膜氧合组（C组）和西沙必利联合腹膜氧合组（D组）。急性肺损伤模型制备成功后4h取右下肺叶组织，采用半定量RT-PCR法测定CC16 mRNA的表达。结果与A组和B组比较，C组和D组CC16 mRNA表达增加（P〈0．01）。与C组比较，D组CC16 mRNA表达增加（P〈0．05）。病理结果显示D组肺组织损伤程度最轻。结论西沙必利联合腹膜氧合可上调油酸致急性肺损伤兔肺组织CC16 mRNA的表达，有利于急性肺损伤的治疗。

Objective To evaluate the effects of cisapride combined with transperitoneal oxygenation on the Clara cell protein （CC16） mRNA in the lung in a rabbit model of oleic acid induced acute lung injury （ALI）. Methods Twenty-four adult rabbits of both sexes weighing 2.0-2.5 kg were used in this study. The animals were tracheotomized, intubated and mechanically ventilated with high frequency jet ventilation under ketamine-diazepam anesthesia. After 30 min of stabilization ALl was induced with oleic acid 0.08 ml/kg injected i.v. over 2 min. ALI was considered successfully induced when PaO2 was reduced to less than 60 mmHg. The animals were randomly divided into 4 groups （ n = 6 each） ： group A ALl; group BALI ＋ cisapride; group CALI ＋ transperitoneal oxygenation and group D ALI＋ cisapride ＋ transperitoneal oxygenation. In group B and D cisapride lmg in 1 ml was given via gastric tube at 0 and 60 min after ALI was successfully induced. In group C and D transperitoneal oxygenation was performed with carbonic amide hydrogen peroxide 100 ml. The first 30 ml was infused transperitoneally at 180 ml/h and the rest 70 ml at 84 ml/h. The animals were killed at 4 h after ALl was induced. The lungs were immediately removed for determination of CC16 mRNA expression （by RT-PCR）. Results CC16 mRNA expression in the lung was significantly higher in group C and D than in group A and B （P 〈 0.05）. CC16 mRNA expression in group D was significantly higher than that in group C （P 〈 0.05）. Microscopic examination showed that the lung injury was slightest in group D. Conclusion Transperitoneal oxygenation combined with cisapride can increase the CC16 mRNA expression in the lung acutely injured in rabbits and is beneficial for the treatment of ALl.