乙型肝炎病毒体外影响骨髓粒系祖细胞集落形成能力的研究

IN VITRO STUDY OF EFFECT OF HBV ON CFU-GM COLONY FORMING

在体外用乙型肝炎病毒（HBV）攻击人骨髓细胞，可引起骨髓粒系祖细胞（CFU—GM）集落形成抑制，其抑制程度与病毒含量呈正相关，加热灭治病毒可消除抑制作用。骨髓细胞内HBVDNA的POR分析显示：40％骨髓细胞感染了HBV，提示骨髓细胞为HBV复制的另一场所，HBV可直接抑制骨髓细胞的分化增殖。

In vitro,human bone marrow cells being challenged with HBV virus results in a dose-dependent suppres-sion of CFU -GM colony forming. Heat inactivation removed the inhibitory effect of HBV on CFU- GM. Polymerase chain reaction(PcR) analysis of HBVDNA in bone marrow cells show that bone marrow cells are another area of HBV replication,HBV can suppress differentiation and devolopment of bone marrow cells.