褪黑素对自身免疫性肝炎的作用研究

The experimental study of the effect of melatonin on rats with autoimmune hepatitis

目的研究褪黑素(MT)对自身免疫性肝炎大鼠模型外周血CD_4^+细胞、肝细胞特异性脂蛋白(LSP)抗体水平及辅助性淋巴细胞(Th)细胞因子水平的影响,并探讨三者之间的相关性。方法制作自身免疫性肝炎大鼠模型,随机分为模型组、MT注射组及猪促肝细胞生长素(pHGF)注射组,60 d后检测各组动物的外周血淋巴细胞亚群浓度、LSP抗体水平及Th细胞因子的浓度.并分析三者之间的关系。结果MT注射组动物CD_4^+细胞百分比、LSP抗体水平、白细胞介素4(IL-4)及白细胞介素6(IL-6)均显著低于模型对照组动物,而白细胞介素2(IL-2)及γ干扰素(IFN-γ)均低于模型对照组。结论外周血CD_4^+百分比及IL-4、IL-6、IL-2、INF-γ和LSP抗体浓度之间存在一定的相关性,其变化均与肝组织病变有关。

Objective To study the influeno of melatonin（MT） on the ovel of scram CD4^＋ cells,anti-liver- specific-protein antibody（anti-LSP） and Th cytokine of rats with autoimmune hepatitis（AIH） ,and to study the relationship among them. Methods AIH model rats were divided randomly into model group, MT group and pig hepacyte growth/aetor（pHGF） group,the serum CD4^＋ ,anti-LSP and Th cytokine after 60 days were detected and their relationships were analysed. Results The percentage of CD4^＋ and the level of IL-4, IL-6, anti-LSP of MT group were much lower than those of model groups, but IL-2 and IFN-γ were higher than model group. Conclusion There＇re certain relatiouships among the percentage of CD4^＋ and the level of IL-4, IL-6, IL-2,anti-LSP and IFN-γ they are all related to pathological changes of hepatic tissue.