他扎罗汀和窄谱中波紫外线联合对角质形成细胞他扎罗汀诱导基因3mRNA的影响

Synergistic effects of narrow-band UVB combined with tazarotene on inducing tazarotene-induced gene 3 mRNA level in normal human keratinocytes

目的：研究他扎罗汀和窄谱中波紫外线（NB—UVB）对培养的正常人角质形成细胞增殖和他扎罗汀诱导基因3（TIG3）mRNA表达的影响。方法：用0．1～1．0μmol／L他扎罗汀和／或50—100mJ／cm^2 NB—UVB处理正常人角质形成细胞24h后，用MTT法和实时荧光定量RT—PCR法分别检测细胞增殖和TIG3 mRNA水平的改变。结果：0．1～1．0μmol／L他扎罗汀处理角质形成细胞后，可抑制细胞的增殖和上调TIG3 mRNA的水平，且较高剂量（1μmol／L）的他扎罗汀的作用强于较低剂量（0．1μmol／L）的他扎罗汀；NB—UVB单独作用时．未能明显抑制角质形成细胞的增殖和上调TIG3 mRNA的水平；二者联合作用时，对角质形成细胞的增殖抑制作用和TIG3 mRNA的诱导作用更强，且强于二者单独作用。结论：他扎罗汀单独处理可抑制角质形成细胞（KC）的增殖和上调TIG3 mRNA水平，但NB—UVB单独照射无此作用；二者联合作用时可协同抑制角质形成细胞的增殖和上调TIG3 mRNA的水平。

Objective：To investigate the alteration of cell proliferation and tazarotene-induced gene 3 （TIG3） mRNA level in cultured normal human keratinocytes after tazarotene and narrow-band UVB （NB-UVB） combined treatment or alone. Methods：Normal human keratinocytes were exposed to NB-UVB（50-100 mJ/cm^2） and/or followed incubation with tazarotene（0.1-1 μmol/L） for 24 hours,then the proliferation potency of the cells was detected by methyl thiazoleterazolium colorimetric assay and the level of TIG3 mRNA was examined by real-time quantitative reverse transcription polymerase chain reaction （RT-PCR）. Results：Cuhured keratinocytes proliferation was inhibited and TIG3 mRNA level was enhanced by both two doses of tazarotene single treatment but not by NB-UVB irradiation alone. Further,the higher dose of tazarotene showed more effects on inhibiting keratinocyte proliferation and elevating TIG3 mRNA level. Tazarotene-NB-UVB combination showed great effects on inhibiting keratinocyte proliferation and increasing TIG3 mRNA level,which were stronger than those induced separately. Conclusion：Tazarotene incubation single can inhibit keratinocyte proliferation and enhance TIG3 mRNA level,but NB-UVB irradiation alone can＇t. Tazarotene-NB-UVB combination showed synergistic effects on inhibiting keratinocyte proliferation and increasing TIG3 mRNA level.