氯胺酮对脂多糖诱发的大鼠肺泡巨噬细胞一氧化氮及氧自由基生成的影响

Effects of ketamine on nitrogen monoxidum and oxygen Radicals in Lipopolysaccharide-stimulated rat alveolar macrophage in vitro

目的:研究氯胺酮对脂多糖(LPS)刺激的大鼠肺泡巨噬细胞一氧化氮(NO)、羟自由基(.OH)及超氧阴离子(O2.-)生成的影响。方法:体外培养的大鼠肺泡巨噬细胞株NR8383分为对照组(C组),LPS组(L组)及不同浓度氯胺酮预处理组(K1、K2和K3组)。检测细胞培养上清液中NO、.OH及O2.-水平。结果:L组NO、.OH及O2.-水平显著高于C组,K2和K3组的NO、.OH及O2.-水平显著低于L组,K2和K3组间无统计学差异。结论:氯胺酮对LPS所致的NR8383的NO、.OH及O2.-的升高有抑制作用。

Objective：To investigate the effects of ketamine on production of Nitric oxide（NO） ,hydroxy radical（·OH） and superoxide anion （O2^-·） in the rat alveolar macrophage cell line （NR8383） activated by Lipopolysaccharide （LPS）, Methods-Macrophage was distributed into five groups,group C,group L,group K1 ,group K2 and group K3. Group L,K1 ,K2 and K3 were stimulated with LPS, Before stimulation ,Group L,K1 ,K2 and K3 were treated with phosphate buffer solution （PBS）, 10,100 and 1 000 μmol/L ketamine. Group C was treated with PBS. The levels of NO, ·OH and O2^-· in the supernatant were assayed. Results ：The concentration of NO, ·OH and O2^-· in group L was increased significantly（P 〈 0.01 ） when＇compared to Group C. The concentration of NO, ·OH and O2^-· in Group K2,K3 was obviously lower than those of Group L （P 〈 0.05）. There was no statistical difference between K2 and K3 （P 〉 0.05）. Conclusions：Ketamine can inhibit LPS-induced production of NO,·OH and O2^-·.