MOBP基因在注意缺陷多动障碍大鼠前额叶皮层表达的研究

Expression of MOBP in the PFC of Animal Model of ADHD in SHR

目的:探讨注意缺陷多动障碍(ADHD)动物模型SHR大鼠前额叶皮层MOBP基因表达情况,分析其在ADHD病理生理学中的意义。方法:应用RT-PCR、免疫组化、电镜技术检测该基因mRNA、蛋白表达情况以及轴突髓鞘形态变化。结果:RT-PCR结果显示SHR大鼠脑组织中MOBP mRNA表达水平(35.78±6.04)%明显低于正常对照WKY大鼠(69.41±7.43)%(P<0.01);免疫组化结果提示SHR大鼠前额叶皮层髓鞘着色显著变浅、稀疏,着色面积占总面积比例(7.43±3.51)%较对照组WKY(14.39±5.82)%明显减少(P<0.05);电镜观察结果显示,部分SHR大鼠髓鞘放射性结构呈直线性分布。结论:MOBP基因在SHR中的表达下调可能通过参与损害中枢神经系统髓鞘结构和功能,在ADHD病理机制中发挥作用。

Objective：To explore the changes of the myelin-associated oligodendrocytic basic protein （MOBP） gene mRNA and protein expression in the prefrontal cortex （PFC） of SHR/WKY and to analyze the relationship between the MOBP and the etiology and pathophysiology of Attention Deficit Hyperactivity Disorder （ADHD）. Methods：The expressive levels of MOBP in the PFC of SHR and WKY were verified by using RT-PCR technique. The distribution of the MOBP in rat brains was marked by used MOBP- HRP immunohistochemistry （IHC） and the electron microscope were applied as the uhrastructural study on brain tissues, Results ：The levels of MOBP gene mRNA expression（35,78 ± 6.04）% in the PFC of SHR were significantly lower than those（69.41 ±7.43）% in the PFC of WKY rats. At the same time,there was uhrastructural difference in arrangement of the radial component in myelin between two groups. Conclusion：The less of MOBP expression in the SHR may be one of the important pathophysiological bases for ADHD.