内皮抑制素和血管内皮生长因子及碱性成纤维细胞生长因子-2在喉鳞状细胞癌中的表达及临床意义

Expression and clinical significance of Endostatin,vascular endothelial growth factor and fibroblast growth factor basic-2 in laryngeal carcinoma

目的探讨内皮抑制素(endostmin)、血管内皮生长因子(vascular endothelial growthfactor,VEGF)和碱性成纤维细胞生长因子-2(fibroblast growth factor basic-2,FGF-2)在喉鳞状细胞癌(简称鳞癌)组织中的表达和临床意义。方法采用流式细胞术检测50例喉鳞癌、40例癌旁组织、10例正常组织中 Endostatin、VEGF 及 FGF-2的表达。结果 Endostatin、VEGF、FGF-2在喉鳞癌组织中的表达量及阳性表达率与癌旁组织和正常喉黏膜组织相比差异有统计学意义(P 值均<0.05);喉鳞癌组织中的表达量及阳性表达率均高于癌旁组织及正常喉黏膜组织,癌旁组织与正常喉黏膜组织之间的表达量及阳性表达率差异无统计学意义(P 值均>0.05)。Endostatin、VEGF、FGF-2在喉鳞癌组织中的表达量及阳性表达率与淋巴结及临床分期有关,与临床分型、患者年龄及性别无关。结论Endostatin、VEGF 及 FGF-2与喉鳞癌的发生、发展密切相关,并可能影响预后。

Objective To evaluate the expression and clinical significance of Endostatin, vascular endothelial growth factor（VEGF） and fibroblast growth factor basic-2 （FGF-2） in the laryngeal squamous cell carcinoma（ LSCC ）. Methods The expression of Endostatin, VEGF and FGF-2 in 50 specimens of LSCC, 40 specimens of para-carcinoma and 10 specimens of normal laryngeal tissues were examined by Flow cytometry. Results Compared with para-carcinoma and normal laryngeal tissues, the expression level and positive rate of Endostatin, VEGF, FGF-2 in LSCC were diferent in statistics（ P 〈 0. 05 ） ;the expression level and positive rate of endostatin, VEGF, FGF-2 in LSCC are obviously higher than those in para-carcinoma and normal laryngeal tissues. The expression level and positive rate of Endostatin, VEGF, FGF-2 were no diference in statistics between para-carcinoma and normal laryngeal tissues（P 〉 0. 05 ）. The expression level and positive rate of Endostatin, VEGF, FGF-2 in LSCC were associated with lymphoid metastasis and clinical stage,not associated with age, sex and clinical group. Conclusions Endostatin, VEGF and FGF-2 play important role in the incidence, development and prognosis of the LSCC.