摘要
为探讨烧伤血清作用下 PMN-EC 粘附力的改变和 CD11/CD18单抗(mAb)对 PMN 与内皮细胞(EC)粘附力的影响,将烧伤血清与人 PMN 或人脐静脉内细胞(HUVEC)一同孵育,在孵育1,3,6,12,24小时分别应用细胞微管吸吮技术系统测定 PMN-HUVEC 粘附力,并用健康人血清刺激作对照。同时,将与烧伤血清孵育1,6,24小时的 PMN,应用 CD11a/CD18mAb 和 CD11b/CD18mAb 处理后,再测其与HUVEC 粘附力。结果表明:PMN 在烧伤血清刺激后与正常 HUVEC 粘附力迅速升高,1小时达到最高峰,并在24小时内维持此水平。烧伤血清激活的 PMN 与 HUVEC 粘附力可被 CD11a/CD18单抗和CD11b/CD18单抗降低70%～80%。HUVEC 在烧伤血清刺激后与 PMN 粘附力呈持续升高趋势,12小时后达最大值,并在以后12小时内无明显变化。提示:烧伤血清具有促进 PMN-EC 粘附,并在烧伤后PMN-EC 粘附中起重要作用,而且刺激 PMN 后所引起的 PMN-EC 粘附力变化与刺激 EC 后所引起的变化不同。CD11/CD18可部分降低 PMN-EC 粘附力,从而可能减轻 PMN 对 EC 的损害。
Our previous studies have proved that a great number of polymorphonuclear neutrophils (PMN) adhered to endothelial lining and induced endothelial cell (EC) injury.This study was attempted to investigate the change in adhesive force between PMN and EC in response to burn patients' serum within 24 hours and effects of antibody against CD11/CD18 on PMN-EC adhesive force.Burn serum was isolated from 4 burn patients (Ⅲ°20%～50%TBSA)within 48 hours after burn injury.PMNs were isolat- ed from 8 volunteers.Adhesive force between a PMN and a HUVEC was calculated by means of mi- cropipette technique at 1h,3h,6h,12h,24h after PMN or HUVEC incubated with burn serum or normal serum.Five individual PMN-HUVEC pairs were measured for each sample.All data were analyzed statis- tically using the t test.Results:①The adhesive force between a single PMN-HUVEC pair increased sharply after PMN incubated with burn serum,which reached the maximun level at 1h after incubation and remained at this level in the following 23 hours.However,the adhesive force was reduced by 78.5%, 72.5%,75.2% at 1h,6h,24h,respectively after the PMNs were pretreated with CD11/CD18 mAb;② The adhesive force between a single burn serum stimulated-HUVEC and PMN pair increased gradually, reached peak at 12h and remained at this level in the following 12 hours.Conclusions:burn serum could induce PMN-EC adhesion,with related to PMN aggregation in internal organs and EC damage after burn injury.Antibodies against CD11/CD18 could partially block PMN-EC adhesion and might reduce EC in- jury induced by activated PMNs.
