舌癌血清标记物的蛋白质组学研究

Proteomics study of the serum biomarkers in tongue cancer

目的通过运用蛋白质组学技术寻找舌癌血清特异性标记物,为舌癌早期诊断、病程监测以及预后判断提供可靠的血清学指标。方法采用舌癌 Tca8113细胞系体外培养,并移植于裸鼠。以正常裸鼠血清为对照,采用双向凝胶电泳分离技术分析荷瘤小鼠的血清蛋白表达谱,并通过质谱鉴定差异表达蛋白。结果在荷瘤裸鼠血清中分离和鉴定了5个差异表达蛋白,其中鳞状细胞癌抗原1检索得分最高,为97分,定量分析中变化最大,并与舌癌密切相关。结论荷瘤裸鼠血清中鳞状细胞癌抗原1高表达,有望成为舌癌的特异性血清标记物之一。

Objective To search for specific serum biomarkers associated with tongue cancer by means of the serum proteomics technology. Methods The tongue cancer ceils of human tongue cancer cell line Tca8113 were subcutaneously inoculated into nude mice, while control nude mice were injected with phosphate-buffered saline. Serums from these two group of mice were collected for proteomic analysis using two-dimensional gel electrophoresis （2-DE） and mass spectrometry （MS）. Results Comparing the serum 2-DE maps from the tumor-bearing mice with those produced from control mice, we found that squamous cell carcinoma antigen 1 was over expressed only in the serum of tumor-bearing mice. Conclusions The squamous cell carcinoma antigen 1 may be of great potential as the biomarker of tongue cancer and as the potential therapeutic target for gene therapy.