摘要
目的建立牙龈卟啉单胞菌膜泡诱导牙龈上皮细胞炎性反应的体外模型,探讨牙龈卟啉单胞菌在牙周炎中的致病作用。方法用酶联免疫吸附法检测牙龈卟啉单胞菌膜泡对牙龈上皮细胞前列腺素 E_2(prostaglandin E_2,PGE_2)分泌的影响,以实时反转录聚合酶链反应法检测牙龈卟啉单胞菌膜泡对牙龈上皮细胞环氧化物酶(cyclooxygenase,COX)-2和白细胞介素(interleukin,IL)-6、IL-8基因表达的作用。结果牙龈卟啉单胞菌膜泡浓度依赖性地促进了牙龈上皮细胞 PGE_2的分泌,并使 COX-2、IL-6、IL-8的 mRNA 表达水平显著上调。结论牙龈卟啉单胞菌膜泡诱导牙龈上皮细胞发生的细胞炎性反应,可能是牙周炎发生、发展的重要因素。
Objective To establish the model of cellular inflammatory responses of gingival epithelial cells in vitro induced by Porphyromonas gingivalis vesicle and to probe into the pathogenesis of Porphyromonas gingivalis in periodontitis. Methods The effect of Porphyromonas gingivalis vesicle on prostaglandin E2 （PGE2 ） production of gingival epithelial cells was detected by ELISA and the effects of Porphyrornonas gingivalis vesicle on cyclooxygenase-2 （COX-2）, interleukin （IL）-6 and IL-8 mRNA expression in gingival epithelial cells were determined by Real-time reverse transcription-polymerase chain reaction（RT-PCR）. Results Porphyrornonas gingivalis vesicle dose-dependently induced PGE2 production and up-regulated COX-2, IL-6 and IL-8 mRNA expression in gingival epithelial cells significantly. Conclusions Cellular inflammatory responses of gingival epithelial cells induced by Porphyromonas gingivalis vesicle may contribute to the initiation and progression of periodontitis.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2007年第9期564-567,共4页
Chinese Journal of Stomatology
基金
辽宁省教育高等学校科学研究项目计划(202013154)
