癫痫持续状态大鼠脑损害及血清烯醇化酶的变化及意义

The changes of serum neuron specific enolose level and morphology in lithium-pilocarpine-induced status epileptitus in adult rats

目的观察大鼠致痫后血清烯醇化酶（neuron specific enolose，NSE）水平变化及海马组织病理学改变。方法采用氯化锂-匹罗卡品联合腹腔注射制成大鼠癫痫持续状态模型（status epileptitus，SE）。用放射免疫法分别对对照组、地西泮干预组、实验组SE后不同时间点血清中NSE水平及常规病理变化进行观测。结果血清NSE水平在大鼠SE后6h开始升高，24-48h达高峰，72h开始下降，1周后趋于正常；实验组血清NSE水平的动态变化与对照组和干预组比较有统计学意义（P〈0．05），而且常规病理也可见海马区神经元变性、坏死性改变，以CA1、CA3区及颞叶皮层为重。而埘照组和地西泮干预组之间血清NSE水平比较无统计学意义（P〉0．05），也无病理形态学变化。结论大鼠SE后可引起脑组织损害，以CA1、CA3区及颞叶皮层损害为主。血清NSE水平是反映神经元损伤的客观生化指标。

Objective To analyze the changes of serun NSE level and histopathology of hippocampus forma-tion. Methods A lithium-pilocarpine indused status epileptitus（SE） is used. A radioimmunoassay and conventional histopathological method were utilized to measure changes of serum NSE level and to observe morphological changes in hippocampus formation of the control group,diazepam group and the experimental groups at different timepoint after SEinadult rats. Results Serum NSE level increased at 6halter SE,speakingduring 24h-48h,and declined at 72h,but it didn＇t return to base line level within 1 week. Serum NSE levels in the lithium-pilocarpine indused seizure groups were significantly elevanted with those of control group or diazepam group（P〈0. 05）. Futhermore ,status epileptitus in adult rats could result to hippocampal neuronal degeneration and death,especially in subfields of CA1 and CA3. Howhere ,there wasn＇t statistical significance in serum NSE levels and morphological changes between later groups. Conclusion Kinding status epileptitus of adult rats cause neurologic damage,especially in subifields of CA1 and CA3 of hippocampus. Measurement of serum concentration of NSE appears to provide a new useful biochemical marker for brain damage.