豚鼠实验变应性肌炎的药物干预治疗及肌肉病理研究

Effect of medication and muscle pathology study on experimental allergic myositis in guinea pig

目的制作豚鼠多发性肌炎模型并探索其可能发病机制及相对安全可靠的治疗新方法。方法用免疫注射的方法制作动物模型并用强的松和动物类中药天龙(Gecko)进行分组干预对比治疗研究,以观察其症状、肌肉病理及肌酶谱变化的区别。结果免疫注射后所有豚鼠均出现不同程度的肌无力、体重减轻、肌酶增高等变化;肌肉病理表现为横纹结构消失、炎细胞浸润和肌细胞的变性、坏死;免疫组化染色见IgG沉积。结论(1)利用免疫注射的方法成功制作了豚鼠实验变应性肌炎动物模型;(2)豚鼠实验变应性肌炎肌肉病理改变主要为横纹肌呈散在分布或灶性分布的各种程度的肌细胞变性、坏死、再生和炎细胞浸润;间质小血管壁增厚,管腔狭窄甚至闭塞;肌膜、肌内膜和肌束膜中有IgG沉积;(3)动物类中药天龙对实验变应性肌炎具有与强的松相似的、比较明显的治疗修复和营养保护作用。

Objective To produce the experimental allergic myositis （EAM） model in guinea pigs and ex-plore the pathogenesis and new relatively safe treatment policy of polymyositis. Methods Animal model was produced by immunifaction and contrast the different curative effect and muscle pathological change between the groups was administered prednisone and Gecko. Results All guinea pigs after injecting showed myasthenia,marcor and increasing creatases levels. The muscle pathology manifested the transverse striation disappeared,inflammatory celles smeared,myocyte degeneration and necrosis and IgG deposition. Conclusion （1）The guinea pig model of EAM was produced successfully by immunifaction. （2）The general muscle pathology change of EAM in guinea pig is striated muscle cells degeneration,necrosis ,and regeneration diffused or focal distribution in levels and inflammatory celles smeared. The small vessels wall is thickened in interstitium and lumens show stenosis ,even obstructed. IgG deposites in sarolemma ,endomysium and exomysium. （3）Gecko ,a animal served as one kind of traditional Chi- nese medicine ,contrasted to prednisone,possess affinis and evident effects on therapy,reparation,nutrition and protection of EAM.