急性鱼藤酮中毒大鼠α-突触核蛋白与酪氨酸羟化酶的表达

α-synuclein and tyrosine hydroxylase expression in acute rotenone toxicity rats

目的探讨鱼藤酮中毒大鼠α-突触核蛋白与酪氨酸羟化酶免疫活性的变化。方法采用大鼠背部皮下持续注射小剂量鱼藤酮的方法,观察大鼠运动功能和生物学特征的变化。结果接受鱼藤酮注射的大鼠均出现明显的运动功能障碍,最严重表现是运动不能。大部分大鼠的黑质-纹状体酪氨酸羟化酶阳性神经元数目减少,全部大鼠的黑质细胞内均有α-突触核蛋白的过度表达。结论鱼藤酮中毒大鼠可以产生帕金森病的运动障碍及有关病理生理学特征。

Objective To explore the expression of α-synuclein and tyrosine hydroxylase in acute rotenone toxicity rats. Methods Chronic low dose （2-3mg/kg/d） rotenone infusion produces clinical features and biological markers of Parkinson＇s disease （PD） in some rats. Results All rotenone infused rats had significant motor function decline beginning one day after the infusion and progressive worsening in the physical condition until they became severely akinetic. In the substantia nigra of rotenone treated rats had reduced numbers of tyrosine hydroxy-lase （TH） positive neurons and had increased nigral α-synuclein expression. Conclusion Our observations show that even a short duration low dose subcutaneous rotenone infusion can induce clinical and pathological markers of PD in some rats.