摘要
采用PCR-SSCP法对太湖鸡催乳素(PRL)、催乳素受体(PRLR)和促卵泡激素β亚基(FSHβ)基因进行多态性检测,并分析其与开产50%后20周产蛋性状的关系。结果表明:PRL和FSHβpro2的基因型频率都是AA型最高,BB型最低,等位基因频率也是A高于B,FSHβ基因exon3位点没有多态;PRLR只有两种基因型,外显子3是AA型频率高于BB型,外显子6是CC型频率高于DD型,且等位基因A和C的频率分别高于等位基因B和D的频率。太湖鸡PRL、PRLR和FSHβ基因开产50%后20周产蛋性状的不同基因型间差异均不显著(P〉0.05),但是PRLR基因外显子3的开产50%后9~11周蛋重平均数基因型间差异接近显著(P=0.08)。基因间交互作用分析发现,催乳素受体基因外显子3和外显子6有极显著的交互作用(P〈0.01),产蛋量较高的合并基因型AADD可以作为太湖鸡选育的参考。
The PCR-SSCP method was applied to test the polymorphism of prolactin(PRL), prolactin receptor (PRLR) and follicle- stimulating hormone beta (FSHI3) genes in Taihu chicken ,and the relationship between the genotypes of PRL, PRLR and FSHI3 and 20 weeks egg production traits after the age at 50% egg in this population was analyzed. The results showed that genotype AA frequency was higher than that of genotype BB in PRL and FSHI3 pro2 genes, the allele A frequency was higher than B frequency, no polymorphism existed in FSHβexon3 ; There were two genotypes in PRLR, and genotype AA frequency was higher than that of genotype BB in exon3 , genotype CC frequency was higher than that of genotype DD in exon6; the allele A/ C frequency was higher than B/D frequency respectively. No significant association was found for the PRL, PRLP and FSHβ genes with the egg production traits after the age at 50% egg(P〉0.05) . But the P value of the salience test of the egg weight average at 9-11 weeks after the age at 50% egg was 0.08 in PRLRexon3. Significant interactions between PRLRexon3 and PRLRexon6 were detected in gene interaction analysis(P〈0.01),and the incorporative genotype AADD is available to breed TaiHu chicken.
出处
《西北农业学报》
CAS
CSCD
北大核心
2007年第5期11-14,共4页
Acta Agriculturae Boreali-occidentalis Sinica
基金
江苏省农业三项工程项目资助[SX(2003)046]