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大鼠肝移植后早期血清一氧化氮的变化及其意义

Changes and significance of serum nitric oxide levels in the early stage after rat liver transplantation
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摘要 目的探讨大鼠肝移植后早期血清一氧化氮(NO)的变化情况及其意义。方法将SD大鼠随机分成A、B、C三组,进行肝移植,供、受者均为SD大鼠。A组于移植肝恢复血流2 h、B组于移植肝恢复血流4 h、C组于移植肝恢复血流6 h时采取受者的下腔静脉血和左肝叶组织,测定血清丙氨酸转氨酶(ALT)和NO含量,免疫组化法测定移植肝组织中核因子κB p65亚单位(NF-κB p65)的表达,并观察肝组织病理学变化;每组均于移植肝恢复血流时收集5 min的胆汁,测量5 min胆汁分泌量。结果A组的5 min胆汁分泌量为(3.73±1.11)μl,明显高于B组的(2.35±0.92)μl和C组的(2.23±0.81)μl(P<0.05)。A组血清ALT含量为(468±36)IU/L,B组为(619±49)IU/L,C组为(820±65)IU/L,A组明显低于B、C组(P<0.05),B组明显低于C组(P<0.05)。A组血清NO含量为(14.2±1.5)μmol/L,明显高于B组的(10.5±1.2)μmol/L和C组的(10.3±1.1)μmol/L(P<0.05)。A组肝组织中NF-κB p65表达阳性细胞百分率为(23.5±1.9)%,B组为(43.8±3.8)%,C组为(48.6±5.1)%,A组明显低于B、C组(P<0.05)。病理学观察显示,随着移植肝脏再灌注时间的延长,肝组织损伤呈进行性加重。Pearson相关性分析提示,NO与血清ALT水平及NF-κB p65表达呈明显的负相关(r值分别为-0.74和-0.77,P<0.01)。结论移植肝脏再灌注早期,血清NO下降,NF-κB的活性逐渐增强,移植肝脏的功能和组织损伤呈加重趋势。 Objective To investigate the changes and significance of serum nitric oxide levels in the early stage after rat liver transplantation. Methods Male Sprague Dawley rats were used as donors and recipients of orthotopic liver transplantation, and the time of cold preservation and anhepatic phase was 4 h and 25 min respectively. Forty-eight rats were randomly divided into groups A, B and C (n = 16 each). The samples of blood were taken from vena cava and hepatic tissues from left lobe 2 h, 4 h, 6 h in groups A, B and C respectively after liver graft reperfusion. ALT and NO levels in serum were detected, and the expression of NF-κB in hepatic tissue was examined by immunohistochemical technique. Amounts of bile flow in 5 min after liver graft reperfused initially were measured. The pathological changes in liver tissues were observed. Results Amounts of bile flow in 5 min in group A (3. 73 ± 1.11 μl) were greater than those group B (2. 35 ± 0. 92μl) and group C (2. 23 ± 0. 81 μl) (P d0. 05). Serum ALT levels in group A (468 ± 36 IU/L) were lower than those in group B (619 ± 49 IU/L) and group C (820 ± 65 IU/L) (P〈0. 05), and serum ALT levels in group B were lower than those in group C (P〈0. 05). Serum NO levels in group A (14. 2 ± 1.5 μmol/L) were higher than those in group B (10. 5 ± 1.2μmol/L) and group C (10. 3 ± 1.1 μmol/L) (P〈0.05). Percentage of positive cells expressing NF-κB 1065 from hepatic tissues in group A (23. 5 % ± 1.9 %) was lower than that in group B (43. 8 0% ± 3. 8 %) and group C (48. 6 0% ± 5. 1%) (P〈0. 05). Histopathologic examination showed that the injuries in hepatic tissues were aggravated as reperfusion time prolonged, meanwhile, Pearson correlation analysis revealed that the levels of NO were negatively correlated with NF-κB p65 and ALT closely (r: - 0. 77, - 0. 74 respectively, P〈0. 01 ). Conclusion In the early stage after rat liver transplantation, serum NO levels were decreased and the activities of NF-κB enhanced, which resulted in the farther damage of liver function and hepatic tissue structures as reperfusion time prolonged.
出处 《中华器官移植杂志》 CAS CSCD 北大核心 2007年第9期534-536,共3页 Chinese Journal of Organ Transplantation
基金 重庆市教委基金(渝教科2003-7)
关键词 肝移植 一氧化氮 再灌注损伤 大鼠 Liver transplantation Nitric oxide Reperfusion injury Rats
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