四川不同地区慈竹和硬头黄遗传多样性的RAPD分析

RAPD Analysis on Genetic Diversity in Different Areas of Bamboo in Sichuan Province

试验采用RAPD—PCR手段分别对四川省不同地区的慈竹（Neosinocalamus affin）和硬头黄（Bambusa rigida）进行了遗传多样性研究。结果表明，利用改良的SDS法成功地从硅胶干燥的幼嫩竹叶中提取了质量较高的DNA。用随机引物A9、B17、C2、C5、C11、D3、Q12对8个待测样品扩增结果的多态性分析表明：共获得50个扩增位点（DNA扩增片段），42个位点具有多态性，高达84％，平均每条引物扩增出7．1条带，包括6条多态性带。通过Popgen32软件对8个竹样进行遗传距离分析，同一竹种不同地区之间遗传距离约为0．1278—0．6539，这表明不同地区之间存在丰富的遗传多样性；慈竹和硬头黄之间遗传距离较远，为0．4463—0．9163。

The genetic diversity in Neosinocalamus affini and Bambusa rigida were studied by RAPD - PCR in different areas of Sichuan Province. The results showed that the good genomic DNA from the bamboo young leaves dried by silica gel was isolated by improved SDS method. The results of RAPD analysis showed that 50 of amplified locus were obtained. Of them, 42 of amplified locus had polymorphism. The 7. 1 of bands were amplified by one primer. Of them, 6 of bands had polymorphism, The genetic distance among 8 samples was analyzed by Popgen 32. The genetic distance between the same type of bamboo from the different regions ranged from 0. 127 8 to 0. 653 9, It indicated that the genetic diversity was rich for the same type of bamboo in the different areas. However, the genetic distance between Neosinocalamus affini and Bambusa rigida was far from 0. 446 3 to 0. 916 3.