大鼠脑出血周边组织MMP-9、TIMP-1表达对脑水肿的影响

Effect of MMP-9, TIMP-1 Expressions on the Brain Edema around Hematoma Intracerebral Hemorrhage

目的:研究大鼠脑出血周边组织基质金属蛋白酶系(MMPs)的成员明胶酶-9(MMP-9)和内源性基质金属蛋白酶抑制物(TIMP-1)表达对脑水肿的影响。方法:Wister大鼠50只随机分为对照组、出血组,各组又分为6、24、48、72、120h等5个时间点。测定脑组织含水量、脑组织示踪剂伊文思蓝(EB)含量和MMP-9、TIMP-1表达。结果:出血后脑组织含水量在72h、EB含量在48h、MMP-9和TIMP-1表达在48h达到高峰,血脑屏障(BBB)在各时间点均有破坏。结论:出血后MMP-9表达可导致BBB通透性增加,TIMP-1通过抑制MMP-9的表达减轻脑水肿。

Aim ： To study the effect of MMP-9, TIMP-1 expressions on the brain edema around hematoma intracerebral hemorrhage（ICH）. Methods： 50 Wistar rats were randomly divided into control group, ICH group. The time （6 h, 24 h, 48 h, 72 h, 120 h） was established in these two groups. Brain water content and Evens blue （EB） content was determined and the expression of MMP-9, TIMP-1 was detected in the rat brain. Results. Brain water content, EB content and the expression of MMP-9, TIMP-1 arrived at the peak at the time 72h,48h and 48h respectively, the ultra-structure of blood-brain barrier（BBB） was damaged all the time. Conclusion： The expression of MMP-9 around hematoma can increase the penetrating ability of BBB, TIMP- 1 can decrease the brain edema by inhibiting the expression of MMP-9.