带捕集阱顶空气相色谱法测定血液中的四氯乙烯

Determination of tetrachloroethylene in blood by headspace gas-chromatography with adsorption trap methods

目的:为血液中四氯乙烯的测定研究一种简单可靠的方法,从而减少传统分析方法中对血液繁杂的前处理、结果准确性差等缺点。方法:用带捕集阱顶空仪进样,气相色谱法经E lite-5MS柱分离后用FID检测,峰形分离较为理想。结果:四氯乙烯含量在0～1400μg/L呈良好的线性,相关系数为0.9994,回归方程Y=176.54X-4849.81,检测限为25μg/L。在线性范围内,对高中低浓度测定精密度,相对标准偏差分别为1.1%、4.2%、5.6%。并在实际样品中进行加标回收试验,回收率为96.5%～103%。干扰试验证明测定四氯乙烯与其他卤代烃测定互不干扰。结论:此方法操作简便,灵敏度较高,满足血液中四氯乙烯检测的需要。

Objective：For analyzing the tetrachloroethylene in blood which is very easy volatilization,a simple and reliable method was studied.Thereby the shortcoming of traditional method was reduced,such as complex process of pre-treating blood and low accuracy of results.Methods：Blood samples were pretreated by Head space gas instrument with adsorption trap were sampled and injected to gas chromatography,and then through Elite-5MS capillary column separated and was detected by FID monitor.The result showed that the separation and peak shape were good.Results：It had a good linearity when the content of tetrachloroethylene was 0~1400 μg/L,and the relevant coefficient 0.9994,the regression equation Y=176.54X-4849.81.The limit of detection was 25 μg/L.In the range of linearity,the variable coefficients were 1.1%,4.2% and 5.6%.with average recovery rate between 97%~103% at different spiking level.No interference was observed in interference test on tetrachloroethylene with other halogenated hydrocarbon,showing that the specificity of the method was good.Conclusion：This method is simple,fast and highly sensitive.And it can meet the need of detection tetrachloroethylene in blood entirely.