固相萃取反相液相色谱法测定保健食品中淫羊藿甙

Determination of icariin in health food by SPE-HPLC

目的:建立固相萃取高效液相色谱法测定保健食品中淫羊藿甙含量的方法。方法:采用固相萃取技术对样品进行净化,以甲醇+水(体积比为50+50)为流动相,ODS柱分离,用二极管阵列检测器于270 nm处检测。结果:淫羊藿甙在2.80～280μg/m l范围内与色谱峰的峰面积呈良好的线性关系(r=0.9999),回收率为91.1%～94.0%,RSD小于4.2%。结论:方法快速、简便、准确、重现性好,可作为保健食品的质量检验的一个定量方法。

Objective：To establish a method for the determination of icariin in health food by SPE-HPLC.Methods：Icariin in health food was extracted with SPE and determined with ODS column using a mixture of CH3OH-H2O（50：50,V：V） as mobile phase and UV detection at 270 nm.Results：There was a good linear relationship within the range of 2.80-280 μg/ml（r=0.9999）.The range of recovery with standard addition was from 91.1% to 94.0% with RSD 4.2%.Conclusion：The method is rapid,simple,accurate and reproducible.It can be used for quality control of health food.