EPO对视网膜缺血再灌注损伤中WTp53、CyclinD1含量变化的影响

Effect of erythropoietin on expression of WTp53 and CyclinD1 in rat retinal ischemia-reperfusion injury

目的研究促红细胞生成素(erythropoietin,EPO)与视网膜缺血再灌注损伤(retinal ischemical reperfusion injury,RIRI)视网膜组织中野生型p53(wild type p53,WTp53)、CyclinD1的关系,为EPO治疗RIRI提供理论依据。方法前房穿刺加压法制作大鼠RIRI模型,28只大鼠随机分为正常组和手术组,其中手术组大鼠左眼为RIRI组,右眼为治疗组(于再灌注开始即刻玻璃体腔内注射EPO20I U),手术组又按照再灌注后不同时间段分为1h、6h、12h、24h、48h、72h组。SABC免疫组织化学法检测视网膜组织中WTp53、CyclinD1的表达。结果RIRI组视网膜在再灌注6h后可发现有WTp53、CyclinD1的表达,24h达到高峰,48h仍持续强表达,72h表达已明显下降。玻璃体腔内注射EPO后上述因子表达趋势不变,但各时间点治疗组较RIRI组表达强度明显减少(P<0.01)。结论玻璃体腔内注射EPO可以抑制WTp53、CyclinD1表达,这可能是EPO治疗RIRI的机制之一。

Objective To investigate erythropoietin （EPO） on the expression of p53 and CyclinD1 in the experimental retinal isehemia-reperfusion injury （RIRI） ,and provide theoretical basis to the therapeutic value. Methods The rat model of experimental RIRI was made by increasing the intraocular pressure. A total of 28 rats were divided into normal and operative group. The latter was subdivided into 1 hour,6 hours, 12 hours,24 hours,48 hours and 72 hours groups after reperfusion,in which the left eyes of the rats were RIRI groups and the right ones were treatment groups （ EPO 20 IU intraeameral injection ）. The expression of 1953 and CyclinD1 was assessed by strept avidin-biotin complex immunohistochemistry. Results No p53 and CyclinD1 positive cells were found in normal group. At the ischemia-reperfusion groups, the expression of p53 and CyclinD1 began to increase at 6th hour after reperfusion. At 24th hour after reperfusion the expression reached the peak, and went on increasing with the reperfusion time prolonged, and began to decease at 72nd hour. The expression trend of p53 and CyclinD1 were unchanged after vitro injection of EPO, but expressive amount decreased obviously（ P 〈 0.01 ）. Conclusion EPO can inhibit the expression of p53 and CyclinD1 ,and may represent an important therapeutic mechanism for RIRI injury.