健脾补糖对过度训练脾虚大鼠骨骼肌GLUT4和P38活性的影响

Effects of Invigorating Spleen and Supplying Saccharide on Overtrained Rats' Skeletal Muscle GLUT4 and P38 Activity

为了探讨健脾补糖对过度训练大鼠骨骼肌的糖转运和MAPK转导通路中P38活性的影响。通过6周递增大负荷游泳训练,观察过度训练和健脾补糖对胃肠的吸收功能,骨骼肌的糖转运、储备和P38活性的影响。研究表明:1)过度训练脾虚的大鼠的骨骼肌MAPK信号转导系统的P38途径虽然被激活。但骨骼肌的葡萄糖转运和糖储存被低血糖和胰岛素所抑制。因此没有明显改善骨骼肌的肌糖原储备。2)健脾补糖从改善胃肠功能、增加能源物质的摄入、提高抗氧化能力等多靶点激活了P38,使骨骼肌对葡萄糖的转运速度与合成速度均加快,在末次运动后24 h就使肌糖原达到了明显的超量恢复状态。

The study focuses on how invigorating the spleen and supplying saccharide affect glucose transport in the skeletal muscle and P38 activity in MAPK signal transduction system of overtrained rats. All groups of rats are trained by increasing load of swimming for 6 weeks, and to observe effects of invigorating spleen and supplying saccharide on function of the sttomach and intestine, glucose transport and storage in the skeletal muscle and P38 activity. It draws a conclusion that 1）Rats of spleen asthenia sports fatigue activates the P38 pathways in MAPK signal transduction passageway in the skeletal muscle. Meanwhile, hypoglycemia and insulin suppress glucose transport and storage in the skeletal muscle. So it is not very signifi- cant in the muscle glycogen store increase.2）Training with invigorating spleen and supplying saccharine may lead to improve stomach and intestine, intake augnetation of energy source substrates , and enhance saccharide oxidation capability in the multi-target. Besides, it plays an important part in the P38 pathways in the MAPK signal transduction system, which may speed up glucose transport and synthesis in the skeletal muscle, and result in the over-recovery of muscle glycogen.