用铜(Ⅱ)-L-精氨酸配体交换薄层色谱拆分氨基酸对映体

Resolution of Amino Acid Enantiomers by Means of Cu (Ⅱ)-L-Arginine Ligand Exchange Thin -Layer Chromatography

报道了一种新的配体交换薄层色谱拆分氨基酸对映体的方法。以醋酸铜-L-精氨酸 的络合物为配体交换剂，用浸渍的方法吸附在硅胶薄层板上，制成配体交换薄层，用PRIS－ MA优化法选择出展开剂的最佳组成为：甲醇／乙腈／四氢呋喃／水=80：8.2:5.8：6，在此 色谱条件下，十对氨基酸对映体得到良好的分离，D-和L-氨基酸的相对比移值在1．09～ 2．40之间。文中对配体交换薄层的制备方法，样品的分子结构及色谱行为、展开剂的组成对 分离效果的影响都进行了详细讨论。

A new method of ligand exchange thin-layer chromatography for the resolution of amino acid enantiomers has been studied. The silica gel plates treated with L-arginine and copper acetate and a solvent system of MeOH/ACN/THF/H2O = 80/8. 2/5. 8/6 were used for the separation of ten pairs of amino acid enantiomers. Significant chromatographic selec- tivity was obstained with relative Rf values of D- to L-amino acids between 1. 09~2. 4. Ef- fects of different plate preparation methods, sample molecular structure and solvent compo- sitions on resolution performance were studied.