微透析-高效液相色谱法测定病人血清中肌酐含量

Determination of Creatinine in Patient Serum by High Performance Liquid Chromatography With Microdialysis sampling Technique

以微透析作为样品预处理技术，与高效液相色谱分析相结合建立了一个测定血清样品中肌酐浓度的方法。同时考察了温度、灌注流速对微透析取样回收率的影响。微透析（透析膜长 4．0 mm，同心圆式，外径 0．5 mm）取样在 37℃、灌注流速为 2.0μL／min时回收率22．29％，RSD 8．27％。利用这种方法测定病人血清中肌酐浓度，简单、快速、重复性较好。

A method to determine creatinine in patient serum by combination of the micro- dialysis sampling technique with high performance liquid chromatrography is presented. The influences of temperature and perfusion flow-rate On recovery of microdialysis sampling were investigated. The recovery of microdialysis sampling is 22. 29 % and RSD 8. 27％ at the per- fusion flow-rate of 2. 0 μL/min and temperature of 37℃. The method developed provides a fast and simple tool for determination of creatinine in patient serum.