HPLC-ELSD测定五苓散中2种泽泻醇的含量

Determination of Alisol A 24-acetate and Alisol B 23-acetate in Wulingsan by HPLC-ELSD

目的采用HPLC-ELSD测定五苓散中泽泻醇A24-乙酸酯、泽泻醇B23-乙酸酯的含量。方法色谱柱：大连依利特Hy-persilC18柱（4．6mm×250mm，5μm）；流动相：乙腈-水（75：25）；检测器：Alhech2000型蒸发光散射检测器；流速为0．8mL·min^-1；柱温：室温；ELSD气体：高纯氮气；流速为2、0L·min^-1；漂移管温度：82℃。结果在五苓散中测定出的泽泻醇A24-乙酸酯、泽泻醇B23-乙酸酯分别在0．330～1．980μg（r=0.9995），0.624～4.680μg（r=0.9994）内呈良好的线性关系。平均回收率分别为101.4％，99.6％，RSD分别为1.7％，3.0％（n=6）。结论采用本法测定五苓散中泽泻醇A24-乙酸酯、泽泻醇B23-乙酸酯的含量，方法简便、可靠、重复性好。

OBJECTIVE To establish a method for the determination of alisol A24-acetate and alisol B23-acetate in Wulingsan. METHODS A HPLC combined with evaporation light scattering detector（ELSD）was applied. Chromatographic column was Hypersil C18 （4.6 mm×250 ram,5 μm）. The mobile phase consisted of acetonitrile-water（75：25 ）at the flow rate of 0.8 mL·min^-1. The gas flow rate of ELSD was 2. 0 L · min^-1 and the temperature of drift tube was 82 ℃. RESULTS The linear ranges of the two compo- nents were 0. 330 - 1. 980 μg（ r = 0. 999 5, n = 5 ） and 0. 624 - 4. 680 μg（ r = 0. 999 4, n = 5 ） respectively. The average recoveries of the two components in Wulingsan were 101.4% （ RSD = 1.7 % ） and 99. 6% （ RSD = 3.0% ） （ n = 6 ） , respectively. CONCLUSION The method is simple ,practical and reliable to be used for the quality control of the Wulingsan.