硫辛酸修饰电化学活化碳纤维微电极选择性测定神经递质5-羟色胺

Selective Determination of Serotonin With Electrochemically Pretreated Carbon Fibre Microelectrode Modified With Lipoic Acid

硫辛酸修饰电化学活化碳纤维微电极是一种新型的化学修饰碳纤维微电极。碳纤维微电极经适当的电化学方法活化后，对神经递质５－羟色胺（５－ＨＴ）的灵敏度高于其代谢物５－羟吲哚乙酸（５－ＨＩＡＡ），在此基础上，用硫辛酸修饰，显著地提高了电极对５－ＨＴ的响应灵敏度，其线性范围为１×１０－８～１×１０－５ｍｏｌ／Ｌ，检测下限为５×１０－９ｍｏｌ／Ｌ，同时有效地抑制了电极对５－ＨＩＡＡ的响应，并且可完全消除尿酸的干扰。

The electrochemically pretreated carbon fibre microelectrode (CFME) modified with lipoic acid is a novel modified CFME. This electrode can increase the determination sen- sitivity of neurotransmitter serotonin (5-HT ) while effectively inhibit the response of its metabolite 5-hydroxyin doleacetic acid (5-HIAA), and completely eliminate the interference of uric acid. The linear range of 5-HT is 1 × 10-8 mol/L-1 × 10-5 mol/L and the detection limit is 5 × 10-9 mol/L.