摘要
建立了测定乳清蛋白中α-La和β-Lg含量的高效液相色谱分析方法,采用Kromasil C8色谱柱(5μm,250mm×4.6mm),流动相A为0.1%的三氟乙酸-水溶液,流动相B为0.09%的三氟乙酸-乙腈溶液梯度洗脱,流速为0.8mL/min,二极管阵列检测器,检测波长215nm,柱温30℃。外标法定量,α-La和β-Lg两种组分线性关系良好,相关系数分别为0.9998和0.9984,检测限为3μg/mL、10μg/mL。
A method was established for the determination of α- lactalbumin and β-1actoglobulin by reversephase HPLC. Separation and quantification was achieved by using a Kromasil C8 column （250mm×4.6mm, 5μm）, mobile phase of A 0.1% TFA in H20 and B 0.09% TFA in acetonitrile, with gradient eluention. Mobile phase at a flow-rate of 0.8mL/min, temperature 30℃ and monitored with photodiode array detector at 215nm. The quantified analysis of external standard calibration curves was used. There were good linear correlations about the two components. The correlation coefficients were 0.9998 and 0.9984. The detection limits were 3μg /mL and 10μg/mL. This method was simple, fast, accurate and convenient.
出处
《食品科技》
CAS
北大核心
2007年第9期203-206,共4页
Food Science and Technology
