温热联合丝裂霉素、5-氟脲嘧啶对人胃癌细胞的体外效应

EFFECTS OF HYPERTHERMIA COMBINED WITH MITOMYCIN, 5-FLUOROURACIL IN VITRO ON HUMAN GASTRIC CARCINOMA CELL

温热联合化疗、放疗及免疫治疗有协同抗癌作用,并能减轻对正常组织的副作用。本实验选择丝裂霉素C(MMC)及5-氟脲嘧啶(5-Fu)联合温热体外作用于人胃癌细胞株(MGC-803),检测其作用后活癌细胞百分计数、克隆形成率及存活分数,以观察温热联合MMC或5-Fu的细胞毒作用。结果表明:温热联合MMC有协同抗癌作用,且随温度的升高其作用逐渐增强,与单纯加温组比较有显著性差异(P<0.01);而温热联合5-Fu无协同抗癌作用,但与单纯加温组比较,在较低温度(37℃)时有显著性差异(P<0.01))。本实验结果为临床应用提供了依据。

The antitumor activity of hyperthermia combined with mitomycin(MMC) ,5-fluorouracil (5-Fu) was observed in human gastric carcinoma cell line MGC-803. The study was aimed at understanding the percentage of living carcinoma cell,plating efficiency and survival fraction. The results showed that hyperthermia combined with MMC had a synergistic antitumor activity which was enhanced with temperature increasing, but it was not the same as hyperthermia combined with 5-Fu. In comparison with simple hyperthermia,the antitumor activity of hyperthermia combined with 5-Fu was enhanced at lower temperture. This results raises a basis of clinical practice.