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δ-连环素在雌激素替代治疗大鼠小脑中的表达

EXPRESSION OF δ-CATENIN IN THE CEREBELLUM OF RAT WITH ESTROGEN REPLACEMENT
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摘要 目的探讨卵巢切除大鼠小脑中δ-连环素(δ-catenin)的表达特征及雌激素对其表达的影响。方法将大鼠分为正常组、假手术组、雌激素替代组和安慰剂组。卵巢切除后人工注射雌二醇或安慰剂,应用免疫组织化学方法检测δ-连环素在小脑的定位表达;用免疫印迹法做定量分析。结果与假手术组和雌激素替代组相比,免疫组织化学和免疫印迹法均显示安慰剂组大鼠小脑中δ-连环素表达最低,假手术组和雌激素替代组δ-连环素表达明显高于安慰剂组。结论雌激素可上调卵巢切除大鼠小脑δ-连环素的表达。 Objective To investigate the expression of δ-catenin in the cerebellum of ovariectomed rat with estrogen replacement and the estrogen effect on δ-catenin expression in the cerebellum of the rats with ovariectome. Methods The rats were divided into normal group, sham operation group, estrogen replacement treatment group and placebo group. Then the rats were artificially injected estradiol or placebo after ovariectome. The expression of δ-catenin in the cerebellum was detected by immunohistochemical method and was quantitated by Western blotting analyses. Results Immunohistochemical method and Western blotting analyses both displayed the expression of δ-catenin in all the groups, while the expression in placebo group was the lowest among the four groups, the expression of δ-catenin in the groups of rats with estrogen replacement treatment and sham operation was significantly higher than that of placebo group. Conclusion The estrogen may upregulate the expression of δ-catenin in the cerebellum in the rat with ovariectome.
作者 魏建峰 刘冠兰 王芳 刘汉武 王小丽 袁琳娜 宋国颖 刘胜洪
机构地区 华中科技大学同济医学院组织学胚胎学教研室
出处 《解剖学报》 CAS CSCD 北大核心 2007年第5期511-514,共4页 Acta Anatomica Sinica
基金 国家自然科学基金资助项目(30370457)
关键词 δ-连环素 小脑 卵巢切除 雌激素 免疫组织化学 免疫印迹法 大鼠 δ-catenin Cerebellum Ovariectomy Estrogen Immunohistochemistry Western blotting Rat
分类号 R322.81 [医药卫生—人体解剖和组织胚胎学]
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参考文献15

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二级参考文献11

  • 1Kosik KS,Donahue CP,Israely I,et al.Delta-catenin at the synaptic-adherens junction.Trends Cell Biol,2005,15 (3):172-178
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  • 5Kapprell HP,Owaribe K,Franke WW,et al.Identification of a basic protein of Mr 75,000 as an accessory desmosomal plaque protein in stratified and complex epithelia.J Cell Biol,1988,106:1679-1691
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  • 7Israely,Costa RM,Xie CM,et al.Deletion of the neuron-specific protein delta-catenin leads to severe cognitive and synaptic dysfunction.Curr Biol,2004,14(18):1657-1663
  • 8Medina M,Merinescu RC,Overhauser J,et al.Hemizygosity of delta-catenin (CTNND2) is associated with severe mental retardation in cri-du-chat syndrome.Genomics,2000,63:157-164
  • 9Ho C,Zhou J,Medina M,et al.Delta-catenin is a nervous system-specific adherens junction protein which undergoes dynamic relocalization during development.J Comp Neurol,2000,420:261-276
  • 10Huang CM,Miyamato H,Huang RH.The mouse cerebellum from 1 to 34 months:Parallel fibers.Nenrobiol Aging,2005,Oct,27 (Epub ahead of print)

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解剖学报
2007年 第5期

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